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SOIL NITRATE NITROGEN

KCl Extraction / Cd-Reduction Method

Scope and Application

This method involves the quantitative extraction of nitrate (NO3-N) from soils using 2.0 N KCI. Nitrate is determined by reduction to nitrite (NO2-N) via a cadmium reactor, diazotized with sulfanilamide and is coupled to N-(I -Napthyl)-ethylenediamine dihydrochloride to form an azochromophore (red-purple in color) measured spectrophotometrically at 520 nm. The method is readily adapted to manual or automated techniques. The procedure outlined follows that outlined by Keeney and Nelson (1982) for determining nitrate nitrogen with a modification in which 25 mL of KCl and 5.0 g of soil are used instead of 100 mL and 10 g soil. Extending the shaking period to thirty minutes with 2.0 N KCl (Bremner et.al. 1965), permits the simultaneous extraction of ammonium and nitrate. Care must be taken to avoid contamination from filter paper and operator handling. Cadmium is a hazardous material, follow manufacturers recommendations in handling this material. Soil nitrate-nitrogen can be used to predict crop response to nitrogen fertilizers. The method detection limit is approximately 0.5 mg kg-1 (on a dry soil basis) and is generally reproducible ± 6%.

Equipment

1. Analytical balance: 100.0 g capacity, resolution ± 0.01 g.

2. Repipette dispenser, calibrated to 25.0 ± 0.2 ml.

3. Reciprocating horizontal mechanical shaker, capable of 180 oscillations per minute (opm).

4. Extraction vessels and associated filtration vessel.

5. Whatman No. 42 or equivalent highly retentive filter paper.

6. spectrophotometer, autoanalyzer, or flow injection analyzer (FIA) instrument.